Article
[Expression and preliminary study of HCV F protein gene].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Feb 2007
Jiang Chun-mei, Diao Zhen-yu, Cui Guo-xing, Qian Wan-hong, Deng Xiao-zhao, Xu Ke, Ding Wei-liang, Tan Yong-fei, Zhang Yun
Abstract excerpt
AIM: To express HCV F protein gene fragment in E.coli and detect if there is its antibody in HCV patients. METHODS: RNA of the virus identified as genetype 1b was choosed as template, the F protein gene was amplified by RT-PCR. This gene fragment was inserted into plasmid vector pGEM simple T. F...
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