Article
[Cloning and sequencing of E2/NS1 gene from a Chinese genotype III isolate of hepatitis C virus].
Zhonghua yi xue za zhi - 1 Feb 1998
Wu C, Tao Q
Abstract excerpt
OBJECTIVE: To clone E2/NS1 gene from a Chinese genotype III isolate of hepatitis C virus into mammalian expression vector and analyze its primary nucleotide structure. METHODS: Reverse transcription nested-PCR methods were employed for amplification E2/NS1 gene which was cloned into mammalian expression vector pcDNA3 by directed clone. Sequence of inserted gene was analyzed by Sanger's method. RESULTS: E2/NS1...
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