Article
Effect of limbal explant orientation on the histology, phenotype, ultrastructure and barrier function of cultured limbal epithelial cells.
Acta ophthalmologica Scandinavica - 1 Jun 2007
Raeder Sten, Utheim Tor Paaske, Utheim Oygunn Aass, Cai Yiqing, Roald Borghild, Lyberg Torstein, Kjeldsen-Kragh Jens, Ramstad Hanne, Messelt Edvard, Nicolaissen Bjørn
Abstract excerpt
PURPOSE: To compare the histology, phenotype, ultrastructure and barrier function of cultured limbal epithelial cells using two explant culture protocols. METHODS: Epithelial cells were cultured for 16 days from limbal explants, positioned with either the stromal side (stromal group) or the epithelial side (epithelial group) on intact amniotic membranes. The cultured epithelium (n = 56) was examined using light...
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