Article
Following temperature stress, export of heat shock mRNA occurs efficiently in cells with mutations in genes normally important for mRNA export.
Eukaryotic cell - 1 Mar 2007
Rollenhagen Christiane, Hodge Christine A, Cole Charles N
Abstract excerpt
Heat shock leads to accumulation of polyadenylated RNA in nuclei of Saccharomyces cerevisiae cells, transcriptional induction of heat shock genes, and efficient export of polyadenylated heat shock mRNAs. These studies were conducted to examine the requirements for export of mRNA following heat shock. We used in situ hybridization to detect SSA4 mRNA (encoding Hsp70) and flow cytometry to measure the amount of...
Topics
- Cell Nucleus
- Gene Deletion
- HSP70 Heat-Shock Proteins
- Heat-Shock Response
- Heterogeneous-Nuclear Ribonucleoproteins
- Hot Temperature
- Mutation
- Nuclear Pore Complex Proteins
- Nuclear Proteins
- Nucleocytoplasmic Transport Proteins
- RNA Transport
- RNA, Fungal
- RNA, Messenger