Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Fast CYP2E1 genotyping using automated fluorescent detection.

La Medicina del lavoro - 1 Jan 2000

Innocenti Claudia, Accorsi A, Cerreta V, Mantovani Vilma, Violante F S

Abstract excerpt

BACKGROUND: Among polymorphic genes coding for xenobiotic-metabolizing enzymes, the ethanol-inducible CYP2E1 gene (1667 bp) is known to play a major role in the metabolism ofseveral chemicals. OBJECTIVES: In order to apply large-scale genotyping, we explored the use of a Single Nucleotide Primer Extension (SNuPE) assay coupled with automated fluorescent detection to assess the presence of low-frequency CYP2E1*5B...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cytochrome P-450 CYP2E1
Share to DNA Primers
Share to Electrophoresis, Agar Gel

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Fluorescence

Share to Polymerase Chain Reaction

Share to Polymorphism, Restriction Fragment Length

Share to Sensitivity and Specificity

Share to Software

Source

Identifiers

PMID: 17219769

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Fast CYP2E1 genotyping using automated fluorescent detection.PMID 17219769Read ↗

High-speed genotyping of CYP1A2*1F mutation with fluorescent hybridization probes using the LightCycler.Semantic proximity Read ↗

High throughput detection of drug-metabolizing enzyme polymorphisms by allele-specific fluorogenic 5' nuclease chain reaction assay.Semantic proximity Read ↗

Assay for the simultaneous detection of the *1C and *1F alleles of the CYP1A2 gene by real-time polymerase chain reaction and melting curve analysis.Semantic proximity Read ↗

Genotyping of polymorphisms in alcohol and aldehyde dehydrogenase genes by direct application of PCR-RFLP on dried blood without DNA extraction.Semantic proximity Read ↗

Comparative analyses of four different methods of genotyping ALDH2.Semantic proximity Read ↗

A multiplex-PCR/RFLP procedure for simultaneous CYP2E1, mEH and GSTM1 genotyping.Semantic proximity Read ↗

Realtime exonuclease-mediated allelic discrimination (READ): a simple homogeneous genotyping assay for SNPs at the ABC gene loci.Semantic proximity Read ↗

Single nucleotide polymorphism genotyping of ALDH2 gene based on asymmetric PCR and fluorescent probe-mediated melting curves.Semantic proximity Read ↗

Rapid genotyping of human ERCC1 exon 4 polymorphism with fluorescence analysis using fluorophore-labeled hybridization probes and a lightcycler.Semantic proximity Read ↗

A new approach for the rapid detection of common and atypical aldehyde dehydrogenase alleles.Semantic proximity Read ↗

Polymorphism of the 5'-flanking region of the CYP2E1 gene: an association study with alcoholism.Semantic proximity Read ↗

An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.