Article
Shiverer jimpy double mutant mice. V. Correlation of genotype and myelin proteins.
Developmental neuroscience - 1 Jan 1991
Sinclair A, Raz Y, Kirschner D A, Villa-Komaroff L, Wolf M K, Billings-Gagliardi S
Abstract excerpt
We have reexamined the levels of myelin basic protein (MBP) and proteolipid protein (PLP) in the brains of mice bred to carry both the shi/shi and jp/Y hypomyelination defects. The genotype of each putative double mutant was confirmed by direct DNA analysis: shi/shi by Southern blot analysis, and jp/Y by restriction enzyme analysis of polymerase chain reaction-amplified fragments. MBP and PLP levels were assessed...
Topics
- Animals
- Blotting, Southern
- Brain Chemistry
- Crossing Over, Genetic
- DNA Mutational Analysis
- Genotype
- Mice
- Mice, Jimpy
- Mice, Neurologic Mutants
- Myelin Basic Protein
- Myelin Proteins