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Article

Intracellular expression of BPTI fusion proteins and single column cleavage/affinity purification by chymotrypsin.

Protein engineering - 1 Jun 1991

Altman J D, Henner D, Nilsson B, Anderson S, Kuntz I D

Abstract excerpt

The novel and efficient expression system described here produces formerly poorly expressed, proteolytically unstable mutants of bovine pancreatic trypsin inhibitor (BPTI). A new, single column method for the cleavage of a recombinant fusion to BPTI and affinity purification of the BPTI moiety by immobilized chymotrypsin is an integral part of the system. Wild-type and mutant BPTI molecules are expressed in...

Topics

Amino Acid Sequence
Aprotinin
Base Sequence
Chromatography, Affinity
Chymotrypsin
Escherichia coli
Genetic Vectors
Hydrolysis
Inclusion Bodies
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Protein Conformation

Recombinant Fusion Proteins

Source

Identifiers

PMID: 1716367
DOI: 10.1093/protein/4.5.593

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1716367-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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