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Article

Intracellular expression of BPTI fusion proteins and single column cleavage/affinity purification by chymotrypsin.

Protein engineering - 1 Jun 1991

Altman J D, Henner D, Nilsson B, Anderson S, Kuntz I D

Abstract excerpt

The novel and efficient expression system described here produces formerly poorly expressed, proteolytically unstable mutants of bovine pancreatic trypsin inhibitor (BPTI). A new, single column method for the cleavage of a recombinant fusion to BPTI and affinity purification of the BPTI moiety by immobilized chymotrypsin is an integral part of the system. Wild-type and mutant BPTI molecules are expressed in...

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PMID: 1716367
DOI: 10.1093/protein/4.5.593

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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