Article
Genotyping of triallelic SNPs using TaqMan PCR.
Molecular and cellular probes - 1 Jun 2007
Morita Akihiko, Nakayama Tomohiro, Doba Nobutaka, Hinohara Shigeaki, Mizutani Tomohiko, Soma Masayoshi
Abstract excerpt
Methods for analysis of single nucleotide polymorphisms (SNP) are well developed. However, most ready-made SNP genotyping kits (e.g., those that use TaqMan PCR) are based on the assumption that the SNP is biallelic. Thus, most kits are unsuitable for triallelic SNPs. We have experienced difficulty genotyping an SNP using TaqMan PCR. In the present study, we developed a method of genotyping a triallelic SNP...
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