Article
Construction and characterization of an Escherichia coli mutant with a deletion of the metZ gene encoding tRNA (f1Met).
Gene - 15 Jul 1991
Kenri T, Kohno K, Goshima N, Imamoto F, Kano Y
Abstract excerpt
The Escherichia coli metZ gene encoding tRNA (f1Met) was replaced by the chloramphenicol-resistance-encoding gene. The resulting mutant exhibited slightly lower growth rates as compared to the wild type at 37 degrees C or 42 degrees C, but grew apparently slower than the latter at 30 degrees C, i...
Topics
- Blotting, Southern
- Chloramphenicol Resistance
- Chromosome Deletion
- Codon
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Mutation
- RNA, Bacterial
- RNA, Transfer, Met
- Temperature
- Transcription, Genetic
- Transduction, Genetic
- beta-Galactosidase
