Article
SNP genotyping by allele-specific PCR using ENA primers.
Nucleic acids symposium series (2004) - 1 Jan 2005
Koizumi Makoto, Morita Koji, Takagi Miho, Yasumo Hiroaki, Kasuya Atsushi
Abstract excerpt
When we placed a 2'-O,4'-C-ethylene nucleic acid (ENA) residue into primers at the 3' end, or the n-1, n-2 or n-3 position, which included a single nucleotide polymorphism (SNP) site at the 3' end, only primers containing the ENA residue at the n-2 position were read by Taq DNA polymerase for amplification. When we compared n-2-position-modified ENA with the corresponding unmodified DNA primers that are often...
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