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Article

Improvement of single nucleotide polymorphism genotyping by allele-specific PCR using primers modified with an ENA residue.

Analytical biochemistry - 15 May 2005

Koizumi Makoto, Morita Koji, Takagi Miho, Yasumo Hiroaki, Kasuya Atsushi

Abstract excerpt

When we placed an ENA residue into primers at the 3' end, or the n-1, n-2, or n-3 position, which included a single nucleotide polymorphism (SNP) site at the 3' end, only primers containing the ENA residue at the n-2 position were read by Taq DNA polymerase for amplification. The use of the ENA p...

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Identifiers

PMID: 15840502
DOI: 10.1016/j.ab.2005.02.029

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-15840502-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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