Asymmetrically primed selective amplification/temperature shift fluorescence polymerase chain reaction to detect the hemoglobin Constant Spring mutation.

Article

Asymmetrically primed selective amplification/temperature shift fluorescence polymerase chain reaction to detect the hemoglobin Constant Spring mutation.

Blood - 1 Jul 1991

Kropp G L, Fucharoen S, Embury S H

Abstract excerpt

Hemoglobin (Hb) Constant Spring is an alpha-thalassemic hemoglobinopathy that is a major cause of severe alpha-thalassemia in Southeast Asians. The difficulty of diagnosing Hb Constant Spring using standard electrophoretic methods has led to interest in DNA-dependent diagnostic methods. The metho...

Topics

Alleles
Alpha-Globulins
Base Sequence
DNA
Fluorescence
Gene Amplification
Hemoglobins, Abnormal
Humans
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Temperature
Thalassemia

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