Article
Assessment of the in situ tyrosine kinase activity of mutant insulin receptors lacking tyrosine autophosphorylation sites 1162 and 1163.
Molecular endocrinology (Baltimore, Md.) - 1 Feb 1991
Yonezawa K, Roth R A
Abstract excerpt
In the present studies mutant insulin receptors with regulatory tyrosine residues 1162 and 1163 changed to phenylalanines were tested for tyrosine kinase activity. In agreement with prior studies, this mutant receptor was found to exhibit almost no insulin-stimulated exogenous kinase activity when assayed in vitro. In contrast, this mutant receptor was found in situ to have a significant, albeit reduced, ability...
Topics
- Animals
- Binding Sites
- Blotting, Western
- Cell Line
- Cricetinae
- Humans
- Insulin
- Kinetics
- Mutation
- Phosphatidylinositol 3-Kinases
- Phosphorylation
- Phosphotransferases