Article
High-level production and covalent immobilization of Providencia rettgeri penicillin G acylase (PAC) from recombinant Pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability.
Biotechnology and bioengineering - 5 Feb 2006
Senerovic Lidija, Stankovic Nada, Spizzo Patrizia, Basso Alessandra, Gardossi Lucia, Vasiljevic Branka, Ljubijankic Goran, Tisminetzky Sergio, Degrassi Giuliano
Abstract excerpt
A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri(rPAC(P.rett))of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genom...
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