Article
Contribution of W229 to the transglycosylation activity of 4-alpha-glucanotransferase from Pyrococcus furiosus.
Biochimica et biophysica acta - 1 Oct 2006
Tang Shuang-Yan, Yang Sung-Jae, Cha Hyunju, Woo Eui-Jeon, Park Cheonseok, Park Kwan-Hwa
Abstract excerpt
A W229H mutant of 4-alpha-glucanotransferase (4-alpha-GTase) from Pyrococcus furiosus was constructed and its catalytic properties were studied to investigate the role of W229 in the catalytic specificities of the enzyme. Various activities and kinetic parameters were determined for the wild-type and W229H mutant enzymes. The transglycosylation factor and transglycosylation activity of the mutant enzyme markedly...
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