Article
Systematic regulation of the enzymatic activity of phenylacetaldoxime dehydratase by exogenous ligands.
Chembiochem : a European journal of chemical biology - 1 Dec 2006
Kobayashi Katsuaki, Kubo Minoru, Yoshioka Shiro, Kitagawa Teizo, Kato Yasuo, Asano Yasuhisa, Aono Shigetoshi
Abstract excerpt
Phenylacetaldoxime dehydratase from Bacillus sp. OxB-1 (OxdB) contains a heme that acts as the active site for the dehydration reaction of aldoxime. Ferrous heme is the active form, in which the heme is five coordinate with His282 as a proximal ligand. In this work, we evaluated the functional role of the proximal ligand for the catalytic properties of the enzyme by "the cavity mutant technique". The H282G mutant...
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