Article
How does heme axial ligand deletion affect the structure and the function of cytochrome b(562)?
Protein engineering - 1 Jun 2001
Kamiya N, Okimoto Y, Ding Z, Ohtomo H, Shimizu M, Kitayama A, Morii H, Nagamune T
Abstract excerpt
We have recently generated a new mutant of cytochrome b(562) (cytb(562)) in which Met7, one of the axial heme ligands, is replaced by Ala (M7A cytb(562)). The M7A cytb(562) can bind heme and the UV-visible absorption spectrum is of a typical high-spin ferric heme. To investigate the effect of the lack of Met7 ligation on the structural integrity of cytb(562), thermal transition analyses of M7A cytb(562) were...
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- Amino Acid Substitution
- Benzothiazoles
- Binding Sites
- Cytochrome b Group
- Escherichia coli
- Escherichia coli Proteins