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Article

A suppressor analysis of residues involved in cation transport in the lactose permease: identification of a coupling sensor.

The Journal of membrane biology - 1 Jan 2006

Franco Peter J, Matzke Elizabeth A, Johnson Jerry L, Wiczer Brian M, Brooker Robert J

Abstract excerpt

Four amino acids critical for lactose permease function were altered using site-directed mutagenesis. The resulting Quad mutant (E269Q/R302L/H322Q/E325Q) was expressed at 60% of wild-type levels but found to have negligible transport activity. The Quad mutant was used as a parental strain to isolate suppressors that regained the ability to ferment the alpha-galactoside melibiose. Six different suppressors were...

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Identifiers

PMID: 16988863
DOI: 10.1007/s00232-005-7020-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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A suppressor analysis of residues involved in cation transport in the lactose permease: identification of a coupling sensor.PMID 16988863Read ↗

An analysis of suppressor mutations suggests that the two halves of the lactose permease function in a symmetrical manner.Semantic proximity Read ↗

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