Article
Laser scanning cytometry in the characterization of the proapoptotic effects of transiently transfected genes in cerebellar granule neurons.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Nov 2006
Bingham Brendan, Kotnis Smita, McHendry-Rinde Barbara, Shen Ru, Wood Andrew, Kennedy Jeffrey D
Abstract excerpt
BACKGROUND: Low transient transfection efficiency limits the ability to characterize putative proapoptotic gene function in neurons. Laser scanning cytometry (LSC), with its high capacity, medium throughput means of collecting fluorescent emissions from cultured cells, offers an effective technology for scoring cell death in neuronal transfectants. METHODS: Cerebellar granule neurons (CGNs) were transfected with...
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