Article
Contraselectable streptomycin susceptibility determinant for genetic manipulation and analysis of Helicobacter pylori.
Applied and environmental microbiology - 1 Sept 2006
Dailidiene Daiva, Dailide Giedrius, Kersulyte Dangeruta, Berg Douglas E
Abstract excerpt
Many Helicobacter pylori genetic studies would benefit from an ability to move DNA sequences easily between strains by transformation and homologous recombination, without needing to leave a conventional drug resistance determinant at the targeted locus. Presented here is a two-gene cassette that can be selected both (i) against, due to a Campylobacter jejuni rpsL gene (dominant streptomycin susceptibility in...
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