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Article

Aflagellated mutants of Helicobacter pylori generated by genetic transformation of naturally competent strains using transposon shuttle mutagenesis.

Molecular microbiology - 1 May 1993

Haas R, Meyer T F, van Putten J P

Abstract excerpt

Three out of 10 Helicobacter pylori clinical isolates were found to be naturally competent for genetic transformation to streptomycin resistance by chromosomal DNA extracted from a spontaneous streptomycin-resistant H. pylori mutant. The frequency of transformation varied between 5 x 10(-4) and 4 x 10(-6), depending on the H. pylori isolate used. Transposon shuttle mutagenesis based on this natural competence was...

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Identifiers

PMID: 8332066
DOI: 10.1111/j.1365-2958.1993.tb01618.x

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