Article
Genetic mutation analysis by CE with LIF detection using inverse-flow derivatization of DNA fragments.
Electrophoresis - 1 Oct 2006
Sang Fuming, Ren Haixia, Ren Jicun
Abstract excerpt
Inverse-flow derivatization is a novel approach to obtain fluorescent DNA derivatives in DNA analysis based on CE with LIF detection. In the present work, we want to explore the feasibility of the application of this method into the mutation detection based on constant denaturant capillary electrophoresis (CDCE) and SSCP analysis. The DNA fragments were first amplified by PCR using a pair of common primers...
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