Analysis of polymerase chain reaction-product by capillary electrophoresis with laser-induced fluorescence detection and its application to the diagnosis of medium-chain acyl-coenzyme A dehydrogenase deficiency.

doi:10.1016/0021-9673(94)85151-4

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Article

Analysis of polymerase chain reaction-product by capillary electrophoresis with laser-induced fluorescence detection and its application to the diagnosis of medium-chain acyl-coenzyme A dehydrogenase deficiency.

Journal of chromatography. A - 7 Oct 1994

Arakawa H, Uetanaka K, Maeda M, Tsuji A, Matsubara Y, Narisawa K

Abstract excerpt

Capillary gel electrophoresis with laser-induced fluorescence (CGE-LIF) has been developed to detect polymerase chain reaction (PCR) amplified samples. LIF detection was performed using Thiazole Orange as the fluorescent intercalating dye. This method was ca. 100x as sensitive as that with UV det...

Topics

Acyl-CoA Dehydrogenase
Bacteriophage phi X 174
Base Sequence
Capillary Action
DNA
DNA Primers
DNA Restriction Enzymes
DNA, Viral
Electrophoresis, Polyacrylamide Gel
Fatty Acid Desaturases
Fluorescence
Humans
Lasers
Molecular Sequence Data

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PMID: 7981832
DOI: 10.1016/0021-9673(94)85151-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7981832-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Mutation

Polymerase Chain Reaction