Article
N-Methyltransferase involved in gramine biosynthesis in barley: cloning and characterization.
Phytochemistry - 1 Sept 2006
Larsson Kristina A E, Zetterlund Ingvor, Delp Gabriele, Jonsson Lisbeth M V
Abstract excerpt
The indole alkaloid gramine occurs in leaves of certain barley (Hordeum vulgare L.) cultivars but not in others. A gene sequence in barley that earlier was characterized as a jasmonate-induced O-methyltransferase (MT) (EC 2.1.1.6, GenBank accession U54767) was here found to be absent in some barley cultivars and breeding lines that all lacked gramine. The cDNA was cloned and expressed in Escherichia coli and the...
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