Article
Novel detection of restriction fragment length polymorphisms in the human major histocompatibility complex.
Immunogenetics - 1 Jan 1991
Cross S J, Tonks S, Trowsdale J, Campbell R D
Abstract excerpt
Cosmid genomic DNA clones have been used as hybridization probes in genomic Southern blot analysis to define restriction fragment length polymorphisms (RFLPs) in the major histocompatibility complex (MHC). Using 14 different enzymes and three overlapping cosmid clones we have detected six RFLPs in a 100 kilobase (kb) segment of DNA in the class III region extending centromeric of the TNFA gene toward HLA-DR. Four...
Topics
- Alleles
- Blotting, Southern
- Chromosome Mapping
- Cosmids
- DNA
- HLA Antigens
- Haplotypes
- Humans
- Immunophenotyping
- Major Histocompatibility Complex
- Male
- Polymorphism, Restriction Fragment Length