Article
Molecular and enzymatic characterizations of novel bifunctional 3beta-hydroxysteroid dehydrogenases/C-4 decarboxylases from Arabidopsis thaliana.
The Journal of biological chemistry - 15 Sept 2006
Rahier Alain, Darnet Sylvain, Bouvier Florence, Camara Bilal, Bard Martin
Abstract excerpt
We have isolated two cDNAs from Arabidopsis thaliana encoding bifunctional 3beta-hydroxysteroid dehydrogenase/C-4 decarboxylases (3betaHSD/D) involved in sterol synthesis, termed At3betaHSD/D1 and At3betaHSD/D2. Transformation of the yeast ergosterol auxotroph erg26 mutant, which lacks 3betaHSD/D activity, with the At3betaHSD/D1 isoform or with At3betaHSD/D2 isoform containing a C-terminal At3betaHSD/D1...
Topics
- 17-Hydroxysteroid Dehydrogenases
- Arabidopsis
- Carbon
- Carboxy-Lyases
- Catalysis
- Cloning, Molecular
- DNA, Complementary
- Endoplasmic Reticulum
- Gene Silencing
- Models, Chemical
- Molecular Sequence Data
- Phenotype
- Phylogeny
- Sterols
- Nicotiana