Article
Rapid identification of single nucleotide polymorphisms and estimation of allele frequencies using sequence traces from DNA pools.
Poultry science - 1 Jul 2006
Ye X, McLeod S, Elfick D, Dekkers J C M, Lamont S J
Abstract excerpt
Millions of single nucleotide polymorphisms (SNP) are now known in the chicken, but the ones that segregate may differ among populations. The current study characterized a simple method to simultaneously identify SNP in populations and to estimate allele frequencies by using relative peak heights of nucleotides in sequencing traces from DNA pools. In the first experiment, known concentration gradients of alleles...
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