Routes to improving the reliability of low level DNA analysis using real-time PCR

Abstract excerpt

BACKGROUND: Accurate quantification of DNA using quantitative real-time PCR at low levels is increasingly important for clinical, environmental and forensic applications. At low concentration levels (here referring to under 100 target copies) DNA quantification is sensitive to losses during preparation, and suffers from appreciable valid non-detection rates for sampling reasons. This paper reports studies on a...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Molecular Biology Techniques and Applications
• Share to Prenatal Screening and Diagnostics