Article
Analysis by pulsed-field gel electrophoresis of DNA double-strand breakage and repair in Deinococcus radiodurans and a radiosensitive mutant.
International journal of radiation biology - 1 Oct 1991
Grimsley J K, Masters C I, Clark E P, Minton K W
Abstract excerpt
Double-strand break (dsb) induction and rejoining after ionizing radiation was analysed in Deinococcus radiodurans and a radiosensitive mutant by pulsed-field gel electrophoresis. Following 2 kGy, migration of genomic DNA (not restriction cleaved) from the plug into the gel was extensive, but was not observed after 90 min postirradiation recovery. By this time D. radiodurans chromosomes were intact, as...
Topics
- DNA
- DNA Damage
- DNA Repair
- DNA, Bacterial
- Electrophoresis
- Gram-Positive Bacteria
- Mutation
- Radiation Tolerance
