Article
Validation of single real-time TaqMan PCR assay for the detection and quantitation of four major genotypes of hepatitis E virus in clinical specimens.
Journal of medical virology - 1 Aug 2006
Enouf V, Dos Reis G, Guthmann J P, Guerin P J, Caron M, Marechal V, Nicand E
Abstract excerpt
Since the characterization of the genome of the hepatitis E virus (HEV) in 1990, a large genetic diversity has been described. A single real-time reverse transcription (RT)-PCR assay with TaqMan technology has been validated which uses only one set of primers and probe within the ORF2 HEV region (nt 5207-5292) for the detection and quantification of the four major genotypes of HEV. This assay proved to be as...
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