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Article

Development and validation of an improved RT-PCR assay with nested universal primers for detection of hepatitis E virus strains with significant sequence divergence.

Journal of virological methods - 1 Nov 2006

Inoue Jun, Takahashi Masaharu, Yazaki Yasuyuki, Tsuda Fumio, Okamoto Hiroaki

Abstract excerpt

Recent studies revealed that hepatitis E virus (HEV) genomes are more variable than previously thought and well-conserved regions suitable for designing universal primers are limited. In this study, based on alignment of 70 full-length HEV sequences of genotypes 1-4, a part of the ORF2/ORF3 overlapping region was found to be the best target region for PCR amplification of various HEV strains. Using the newly...

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Identifiers

PMID: 16901555
DOI: 10.1016/j.jviromet.2006.07.004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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