Article
Culture of phenotypically defined hematopoietic stem cells and other progenitors at limiting dilution on Dexter monolayers.
Blood - 15 Aug 1991
Weilbaecher K, Weissman I, Blume K, Heimfeld S
Abstract excerpt
Highly enriched, phenotypically defined hematopoietic stem, Thy-1loLin-Sca-1+, and progenitor cell populations from mouse bone marrow (BM) were tested at limiting dilution for their ability to reconstitute Dexter monolayers. Several classes of BM cells can reconstitute Dexter cultures, first forming discrete "cobblestone" areas which then mature into colonies consisting primarily of maturing myeloid and erythroid...
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