Article
Terbium(III) luminescence study of the spatial relationship of tryptophan residues to the two metal ion binding sites of Escherichia coli glutamine synthetase.
Biochemistry - 9 Apr 1991
McNemar L S, Lin W Y, Eads C D, Atkins W M, Dombrosky P, Villafranca J J
Abstract excerpt
The luminescence of Tb(III) was used to explore the topography of the metal ion sites of Escherichia coli glutamine synthetase and the relationship between these sites and tryptophan residues of the enzyme. By irradiation of tryptophan residues at 295 nm and measurement of the resulting Tb(III) luminescence at 544 nm, a biphasic curve was obtained upon titrating apoenzyme with Tb(III) indicating sequential...
Topics
- Binding Sites
- Cloning, Molecular
- Energy Transfer
- Escherichia coli
- Glutamate-Ammonia Ligase
- Luminescent Measurements
- Metals
- Mutation
- Sensitivity and Specificity
- Serine
- Terbium