Article
Amplification by the polymerase chain reaction of hypervariable regions of the human genome for evaluation of chimerism after bone marrow transplantation.
Blood - 1 Apr 1991
Ugozzoli L, Yam P, Petz L D, Ferrara G B, Champlin R E, Forman S J, Koyal D, Wallace R B
Abstract excerpt
We combined the polymerase chain reaction (PCR) with oligonucleotide hybridization as a novel and sensitive technique to evaluate posttransplant chimerism. Specific oligonucleotides for hybridization were synthesized homologous to tandemly repetitive core sequences of regions with a variable numb...
Topics
- Base Sequence
- Bone Marrow Transplantation
- Chimera
- Genetic Variation
- Humans
- Leukemia
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length
- Repetitive Sequences, Nucleic Acid