Article
Real-time PCR quantification of haematopoietic chimerism after transplantation: a comparison between TaqMan and hybridization probes technologies.
International journal of laboratory hematology - 1 Feb 2010
Martínez-López J, Crooke A, Grande S, Ayala R, Jiménez-Velasco A, Gamarra S, Meneu J C, Gilsanz F
Abstract excerpt
This study aimed to compare the sensitivity and accuracy of two methods of quantitative real-time polymerase chain reaction (qrt-PCR), in order to determine haematopoietic chimerism (CH): single nucleotide polymorphisms using TaqMan (TM) probes and insertion/deletion polymorphisms using Hybridization (Hyb) probes. A total of 106 samples from 20 patients who underwent allogenic stem cell transplantation (n = 14)...
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