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Article

Multiplex real-time quantitative polymerase chain reaction assay for rapid and sensitive detection of hematopoietic chimerism.

HLA - 1 Oct 2018

Nadvornikova Sylvie, Leontovycova Monika, Pegova Kristyna, Hrabakova Pavla, Prerovska Renata, Cechova Hana

Abstract excerpt

The increase of mixed chimerism (MC) after allogeneic hematopoietic stem cell transplantation has been associated with a high risk of relapse. A variety of techniques that use polymorphic markers have been established to survey hematopoietic chimerism status. The highest sensitivity is achieved using real-time quantitative polymerase chain reaction (RQ-PCR) analysis of insertion/deletion polymorphism, which...

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PMID: 30129247
DOI: 10.1111/tan.13383

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