Article
Detection of complex alleles by direct analysis of DNA heteroduplexes.
Immunogenetics - 1 Jan 1991
Sorrentino R, Iannicola C, Costanzi S, Chersi A, Tosi R
Abstract excerpt
DNA molecules derived from three alleles of the HLA-DRB3 locus and differing from each other at several nucleotide sites were denatured and cross-hybridized. Each allelic combination was found to generate a pair of heteroduplexes of different mobility. Their retardation as compared to homoduplexes was proportional to the number of mismatches. In each heteroduplexes pair the component possessing the highest number...
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