Detection of complex alleles by direct analysis of DNA heteroduplexes.

doi:10.1007/BF00210825

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Article

Detection of complex alleles by direct analysis of DNA heteroduplexes.

Immunogenetics - 1 Jan 1991

Sorrentino R, Iannicola C, Costanzi S, Chersi A, Tosi R

Abstract excerpt

DNA molecules derived from three alleles of the HLA-DRB3 locus and differing from each other at several nucleotide sites were denatured and cross-hybridized. Each allelic combination was found to generate a pair of heteroduplexes of different mobility. Their retardation as compared to homoduplexe...

Topics

Alleles
Base Sequence
DNA
Electrophoresis, Polyacrylamide Gel
HLA-DR3 Antigen
Histocompatibility Testing
Humans
Molecular Sequence Data
Nucleic Acid Conformation
Nucleic Acid Heteroduplexes
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length

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