Article
Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation.
Biological chemistry - 1 Mar 2006
Neschastnova Anna A, Gasanova Victoria K, Popenko Vladimir I, Lambrinakos Andreana, Belitsky Gennady A, Cotton Richard G H, Yakubovskaya Marianna G
Abstract excerpt
Mutation detection and mismatch repair investigations based on heteroduplex formation require a linear DNA structure. DNA branching, described previously under physiological conditions, has been analysed in the heteroduplex formation process. Symmetrical chi-structures were detected after heteroduplex formation by gel electrophoresis and electron microscopy. Buffer composition, DNA concentration and duplex...
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