Article
Genetic diagnosis of band 3 deficiency and sexing in bovine preimplantation embryos.
The Journal of veterinary medical science - 1 Apr 2006
Kageyama Soichi, Hirayama Hiroki, Moriyasu Satoru, Inaba Mutsumi, Ito Daisuke, Ohta Hiroshi, Sawai Ken, Minamihashi Akira, Onoe Sadao
Abstract excerpt
Band 3 deficiency with hereditary spherocytosis and hemolytic anemia in Japanese black cattle, band 3(Bov.Yamagata), is caused by a total lack of band 3 protein with an autosomal dominant inheritance. Genotyping for band 3 deficiency and sexing were successfully achieved in biopsied embryo cells with efficiencies of 98.4% and 97.4%, respectively. Transfer of the embryo that was determined as homozygous for the...
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