Article
Cell-cycle dependent mutation of human lymphoblasts: bromodeoxyuridine and butyl methanesulfonate.
Mutation research - 1 Jan 2000
Hoppe H, Call K M, Leong P M, Thilly W G
Abstract excerpt
Cells of the human lymphoblast line WI-L2 and its derivative TK-6 were synchronized by centrifugal elutriation and cell-cycle dependent mutation to 6TGR (HPRT) and OUAR (Na+, K+ ATPase) measured. Bromodeoxyuridine induced 6TGR and OUAR mutations within S phase while butylmethyl-sulfonate induced mutation displayed no cell-cycle dependence. The data indicate that centrifugal elutriation is a facile means to obtain...
Topics
- Bromodeoxyuridine
- Cell Cycle
- Cell Division
- Cell Line
- Centrifugation
- DNA
- Lymphocytes
- Mesylates
- Mutagens
- Mutation