Article
Glycosylation of asparagine 24 of the natriuretic peptide receptor-B is crucial for the formation of a competent ligand binding domain.
Molecular and cellular biochemistry - 1 Aug 1997
Fenrick R, Bouchard N, McNicoll N, De Léan A
Abstract excerpt
UV cross-linking studies of the natriuretic peptide receptor-B (NPR-B) using radiolabeled C-type natriuretic peptide (CNP) indicate that only fully glycosylated receptors are capable of binding ligand. We therefore used site-directed mutagenesis to determine which potential glycosylation sites ar...
Topics
- Amino Acid Sequence
- Animals
- Asparagine
- Binding, Competitive
- Blotting, Western
- COS Cells
- Carbohydrate Metabolism
- Cattle
- Cross-Linking Reagents
- Cyclic GMP
- Glycosylation
- Guanylate Cyclase