Article
Nucleotide sequence of the Escherichia coli rfe gene involved in the synthesis of enterobacterial common antigen. Molecular cloning of the rfe-rff gene cluster.
The Journal of biological chemistry - 15 Jan 1992
Meier-Dieter U, Barr K, Starman R, Hatch L, Rick P D
Abstract excerpt
The genetic determinants of enterobacterial common antigen (ECA) include the rfe and rff genes located between ilv and cya near min 85 on the Escherichia coli chromosome. The rfe-rff gene cluster of E. coli K-12 was cloned in the cosmid pHC79. The cosmid clone complemented mutants defective in the synthesis of ECA due to lesions in the rfe, rffE, rffD, rffA, rffC, rffT, and rffM genes. Restriction endonuclease...
Topics
- Amino Acid Sequence
- Antigens, Bacterial
- Base Sequence
- Cloning, Molecular
- Cosmids
- Electrophoresis, Polyacrylamide Gel
- Enterobacteriaceae
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
