Article
Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus.
Journal of bacteriology - 1 Sept 1990
Austin E A, Graves J F, Hite L A, Parker C T, Schnaitman C A
Abstract excerpt
Tn10 insertions were selected on the basis of resistance to the lipopolysaccharide (LPS)-specific bacteriophage U3. The majority of these were located in a 2-kilobase region within the rfa locus, a gene cluster of about 18 kb that contains genes for LPS core biosynthesis. The rfa::Tn10 insertions...
Topics
- Carbohydrates
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Lipopolysaccharides
- Mutation
- Operon
- Plasmids
- Protein Biosynthesis
- Restriction Mapping
- Transcription, Genetic
- Transduction, Genetic