Article
A voltage-dependent gating transition induces use-dependent block by tetrodotoxin of rat IIA sodium channels expressed in Xenopus oocytes.
Neuron - 1 Oct 1991
Patton D E, Goldin A L
Abstract excerpt
We have utilized molecular biological techniques to demonstrate that rat IIA sodium channels expressed in Xenopus oocytes were blocked by tetrodotoxin (TTX) in a use-dependent manner. This use dependence was the result of an increased affinity of the channels for TTX upon depolarization, most likely due to a conformational change in the channel. Using a mutant with a slower macroscopic rate of inactivation, we...
Topics
- Animals
- Electrophysiology
- Ion Channel Gating
- Mutation
- Oocytes
- Sodium Channels
- Tetrodotoxin
- Xenopus