Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Site-directed PEGylation of human basic fibroblast growth factor.

Protein expression and purification - 1 Jul 2006

Wu Xiaoping, Li Xiaokun, Zeng Yaoying, Zheng Qing, Wu Sixian

Abstract excerpt

Through site-directed mutagenesis, three cysteines of human basic fibroblast growth factor (hbFGF) were replaced with serine residues, resulting in a hbFGF mutant named hbFGFSer25,69,92. The mutant with only one cysteine residue at the 87th position, whose mitogenic activity was comparable to that of wild-type hbFGF, was further coupled to polyethylene glycol with a molecular size of 5 kDa (PEG5K) via the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cysteine
Share to Fibroblast Growth Factor 2
Share to Mice

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Mutagenesis, Site-Directed

Share to Mutation

Share to Polyethylene Glycols

Share to Recombinant Proteins

Share to Serine

Source

Identifiers

PMID: 16545577
DOI: 10.1016/j.pep.2006.02.002

Genes

SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Site-directed PEGylation of human basic fibroblast growth factor.PMID 16545577Read ↗

Enhancement of the pharmacological efficacy of FGF-21 by genetic modification and PEGylation.Semantic proximity Read ↗

Development of a human epidermal growth factor derivative with EGFR-blocking and depleted biological activities: A comparative in vitro study using EGFR-positive breast cancer cells.Semantic proximity Read ↗

Conversion of cysteine to serine residues alters the activity, stability, and heparin dependence of acidic fibroblast growth factor.Semantic proximity Read ↗

Site-directed pegylation of recombinant interleukin-2 at its glycosylation site.Semantic proximity Read ↗

Lysine-deficient lymphotoxin-α mutant for site-specific PEGylation.Semantic proximity Read ↗

A novel high-throughput screening method for microbial transglutaminases with high specificity toward Gln141 of human growth hormone.Semantic proximity Read ↗

PEGylation of a glycosaminoglycan-binding, dominant-negative CXCL8 mutant retains bioactivity in vitro and in vivo.Semantic proximity Read ↗

Site-specific PEGylation of a mutated-cysteine residue and its effect on tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL).Semantic proximity Read ↗

Purification and characterization of the 210-amino acid recombinant basic fibroblast growth factor form (FGF-2).Semantic proximity Read ↗

[Effect and mechanism of action of non-mitogenetic human acidic fibroblast growth factor on the mitogenic activity of the mammary tumor cell].Semantic proximity Read ↗

Site-directed mutagenesis of heparin-binding EGF-like growth factor (HB-EGF): analysis of O-glycosylation sites and properties.Semantic proximity Read ↗

Identification of diphtheria toxin R domain mutants with enhanced inhibitory activity against HB-EGF.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.