Article
Strategies to obtain stable transgenic plants from non-embryogenic lines: complementation of the nn1 mutation of the NORK gene in Medicago sativa MN1008.
Plant cell reports - 1 Aug 2006
Perhald Ariana, Endre Gabriella, Kevei Zoltan, Kiss Gyorgy B, Kereszt Attila
Abstract excerpt
Strategies to introduce genes into non-embryogenic plants for complementation of a mutation are described and tested on tetraploid alfalfa (Medicago sativa). Genes conditioning embryogenic potential, a mutant phenotype, and a gene to complement the mutation can be combined using several different crossing and selection steps. In the successful strategy used here, the M. sativa genotype MnNC-1008(NN) carrying the...
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