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Article

Transformation of the plant Kalanchoë daigremontiana using Agrobacterium tumefaciens.

Cold Spring Harbor protocols - 1 Oct 2009

Garcês Helena, Sinha Neelima

Abstract excerpt

Kalanchoë daigremontiana can be stably transformed using the Agrobacterium tumefaciens-mediated T-DNA transfer method, as described here. Sterilized plant tissue is cocultivated with an A. tumefaciens suspension, transformants are selected and the shoots are grown in rooting medium and then in soil. Plant phenotypes can be examined approximately 3 mo after transfer of plants to soil.

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Identifiers

PMID: 20147048
DOI: 10.1101/pdb.prot5303

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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