Article
Virus-induced gene silencing (VIGS) as a reverse genetic tool to study development of symbiotic root nodules.
Molecular plant-microbe interactions : MPMI - 1 Jun 2008
Constantin G D, Grønlund M, Johansen I E, Stougaard J, Lund O S
Abstract excerpt
Virus-induced gene silencing (VIGS) can provide a shortcut to plants with altered expression of specific genes. Here, we report that VIGS of the Nodule inception gene (Nin) can alter the nodulation phenotype and Nin gene expression in Pisum sativum. PsNin was chosen as target because of the distinct non-nodulating phenotype of nin mutants in P. sativum, Lotus japonicus, and Medicago truncatula. The vector based...
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