Article
Biolistic transformation of grapevine using minimal gene cassette technology.
Plant cell reports - 1 Aug 2006
Vidal Jose R, Kikkert Julie R, Donzelli Bruno D, Wallace Patricia G, Reisch Bruce I
Abstract excerpt
The use of minimal gene cassettes (MCs), which are linear DNA fragments (promoter+open reading frame+terminator) lacking the vector backbone sequence, was compared to the traditional use of whole circular plasmids (CPs) for transformation of grapevine. Embryogenic cell suspensions of 'Chardonnay' (Vitis vinifera L.) were transformed via particle co-bombardment using two nonlinked genes in either MCs or CPs. One...
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