Article
Use of randomly mutagenized genomic cDNA banks of potato spindle tuber viroid to screen for viable versions of the viroid genome.
The Journal of general virology - 1 Feb 2011
Więsyk Aneta, Candresse Thierry, Zagórski Włodzimierz, Góra-Sochacka Anna
Abstract excerpt
In an effort to study sequence space allowing the recovery of viable potato spindle tuber viroid (PSTVd) variants we have developed an in vivo selection (Selex) method to produce and bulk-inoculate by agroinfiltration large PSTVd cDNA banks in which a short stretch of the genome is mutagenized to saturation. This technique was applied to two highly conserved 6 nt-long regions of the PSTVd genome, the left...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
